ZHANG Guiqing;LIANG Zhihao;LIU Qing;WU Xiaoping;LUO Hailing;YAO Junxin;LIU Bin;LIN Zhanxi;JIANG Shusong;National Research Center for Juncao Engineering, Fujian Agriculture and Forestry University;College of Food Science, Fujian Agriculture and Forestry University;Fujian Chanyi Tea Path Agricultural Comprehensive Development Co., Ltd.;In order to explore the water extraction process and quality standard of compound Juncao Ganoderma lucidum granules, liquid extract yield and liquid extract polysaccharide content were taken as indexes, the effects of extraction temperature, extraction time, ratio of material to liquid, extraction times and soaking time on extraction yield and polysaccharide content were investigated by single factor and response surface experiments. The particle size, moisture content, weight loss on drying, loading difference, solubility, microbial limit and heavy metal content of granules were detected according to the 2015 edition of Pharmacopoeia of the People's Republic of China and the national standard. The raw materials of Juncao Ganoderma lucidum, Perilla frutescens and selenium-enriched tea in compound Juncao Ganoderma lucidum granules were identified by thin layer chromatography, and the contents of monosaccharides and tea polyphenols in compound Juncao Ganoderma lucidum granules were determined through high performance liquid chromatography. The results showed that the optimal extraction conditions were as follows: extraction temperature 80 ℃, extraction time 2.5 h, ratio of material to liquid(g/mL) 1∶25, two times of extraction, and immersion time 6 h. The particle size, water content, solubility, weight loss on drying and loading difference of the granules reached the requirements of the 2015 edition of Pharmacopoeia of the People's Republic of China.The microbial limit and heavy metal content of the granules met the requirements of the national standard, and the average selenium content was 0.058 3 mg/kg. The TLC bands of Juncao Ganoderma lucidum, Perilla frutescens and selenium-enriched tea were clear and well separated, with no interference from the negative control. The contents of mannose, rhamnose, glucose, galactose, arabinose, epigallocatechin gallate, epicatechin gallate, catechin, gallic acid in compound Juncao Ganoderma lucidum granules were 1.310, 1.166, 8.708, 1.265, 0.824, 5.79, 2.58, 1.72 and 1.67 mg/g, respectively. The optimized water extraction process of the compound Juncao Ganoderma lucidum granules is stable and reliable, all the indexes of the granules meet the requirements, and the established quality standard for the water extraction process of compound Juncao Ganoderma lucidum granules is feasible.
2025 03 v.53;No.219 [Abstract][OnlineView][Download 2967K]